CCL-25 WISH 人羊膜細(xì)胞.

ATCC® Number: CCL-25™ Price: $323.00

Additional information about this cell line

Designations: WISH

Depositors: L Hayflick

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

CCL-25 WISH 人羊膜細(xì)胞.

Source: Organ: HeLa contaminant

Cellular Products: keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Susceptibility: poliovirus 1, 2; adenovirus 3; vesicular stomatitis （Indiana）

Reverse Transcript: negative

DNA Profile （STR）: Amelogenin: X

CSF1PO: 9,10

D13S317: 13.3

D16S539: 9,10

D5S818: 11,12

D7S820: 8,12

THO1: 7

TPOX: 8,12

vWA: 16,18

Isoenzymes: G6PD, A

HeLa Markers: Y

Comments: This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.

The cells are positive for keratin by immunoperoxidase staining.

NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination

Propagation: ATCC complete growth medium: Minimum essential medium （Eagle） in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22153: Hayflick L. The establishment of a line （WISH） of human amnion cells in continuous c*tion. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490

26034: . . Am. J. Dis. Child. 103: 460, 1962.

CCL-25 WISH人羊膜細(xì)胞。

ATCC®編號：CCL-25™價(jià)格：323.00美元

有關(guān)此細(xì)胞系的其他信息

名稱：WISH

存款人：L Hayflick

生物安全級別：2

運(yùn)送：冷凍

中等和血清：見傳播

生長特性：貼壁

生物體：智人（人）

形態(tài)：上皮

CCL-25 WISH

CCL-25 WISH人羊膜細(xì)胞。

來源：器官：HeLa污染物

蜂窩產(chǎn)品：角蛋白

許可證/表格：除上述MTA之外，轉(zhuǎn)移此ATCC材料可能需要其他ATCC和/或管理許可證。購買ATCC材料的任何人終都需要負(fù)責(zé)獲得許可。請點(diǎn)擊此處了解有關(guān)裝運(yùn)到您所在地的具體要求的信息。

病毒易感性：脊髓灰質(zhì)炎病毒1,2;腺病毒3;水泡性口炎（印第安納州）

反轉(zhuǎn)錄：否定

DNA圖譜（STR）：釉原蛋白：X.

CSF1PO：9,10

D13S317：13.3

D16S539：9,10

D5S818：11,12

D7S820：8,12

THO1：7

TPOX：8,12

vWA：16,18

同工酶：G6PD，A

HeLa標(biāo)記：Y

評論：這條線

zi初被認(rèn)為是源自正常的羊膜，但后來發(fā)現(xiàn)，基于同工酶分析，HeLa標(biāo)記染色體和DNA指紋分析已經(jīng)通過HeLa細(xì)胞污染建立。

通過免疫過氧化物酶染色，細(xì)胞對角蛋白呈陽性。

注意：此行的細(xì)胞含有HeLa標(biāo)記染色體，并且是通過HeLa污染衍生的

繁殖：ATCC*生長培養(yǎng)基：Earle's BSS中的低必需培養(yǎng)基（Eagle），含有非必需氨基酸和1mM丙酮酸鈉，90％;胎牛血清，10％

溫度：37.0℃

傳代培養(yǎng)：傳代培養(yǎng)比例：建議傳代比例為1：2至1:10

中等更新：每周兩次

取出培養(yǎng)基，加入新鮮的0.25％胰蛋溶液3到5分鐘，去除，讓培養(yǎng)物在室溫下靜置5-10分鐘。加入新鮮培養(yǎng)基，吸入并分裝到新的培養(yǎng)瓶中。

保存：培養(yǎng)基95％; DMSO，5％

相關(guān)產(chǎn)品：推薦培養(yǎng)基（不含ATCC培養(yǎng)基描述的額外補(bǔ)充劑或血清）：ATCC 30-2003

推薦血清：ATCC 30-2020

參考文獻(xiàn)：22153：Hayflick L.在連續(xù)培養(yǎng)中建立人羊膜細(xì)胞系（WISH）。進(jìn)出口。細(xì)胞研究。 23：14-20,1961。PubMed：13712490

26034：。 。上午。 J. Dis。兒童。 103：460,1962。