CRL-5971 SNU-1 人胃癌細(xì)胞

ATCC® Number: CRL-5971™ Price: $417.00

Designations: SNU-1

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens （human）

Morphology: epithelial

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

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CRL-5971 SNU-1 人胃癌細(xì)胞

Isolation: Isolation date: 1984

Receptors: vasoactive intestinal peptide （VIP）, expressed

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type O; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen （CEA） and TAG 72.

DNA Profile （STR）: Amelogenin: X,Y

CSF1PO: 12,13

D13S317: 11,14

D16S539: 9,12

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,10

vWA: 14

Cytogenetic Analysis: SNU-1 is a near-diploid cell line with a modal chromosome number of 47; double minute chromosomes were found in 28% of the cells. They have a reported colony-forming efficiency of 1.9% in semisolid medium and a population doubling time of 26 hours. The cells grow as floating aggregates of round cell clusters. This is a hyperdiploid human cell line with the modal chromosome number of 47 occurring in 94% of cells. Higher ploidies occurred at 42%, which is very high. The karyotype of 47,XY,t（1;4） （q24;q33）, +M1 is very uniform and stable. M1 is an F- sized small metacentric chromosome.

Age: 44 years adult

Gender: male

Ethnicity: Asian

Comments: SNU-1 was derived in 1984 by J. Park and associates from a poorly differentiated primary carcinoma of the stomach, taken prior to cytotoxic therapy. The cells are L-dopa decarboxylase （DDC） negative. SNU-1 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of c-myc and c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 26 hrs

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

CRL-5971 SNU-1 人胃癌細(xì)胞